huvecs complete culture medium  (Procell Inc)

Journal: Bioactive Materials

Article Title: Self-assembled hybrid hydrogel microspheres create a bone marrow-mimicking niche for bone regeneration

doi: 10.1016/j.bioactmat.2025.08.003

Figure Lengend Snippet: Angiogenesis effect of the self-assembled hybrid microspheres-based BM-mimicking niche. A-E) Results from the groups without BMSCs: A, B) Representative Transwell migration images and the quantitative assessment of HUVECs numbers based on the crystal violet staining results on day 1. C, D) Representative scratch assay images and the quantitative analysis of migration rate of HUVECs on day 1. E-F) Representative images and quantitative assessment of tube-like structure formation of HUVECs induced by released peptides at 6 h. G-H) Representative images and quantitative assessment of tube-like structure formation of HUVECs cultured in the conditioned medium from surface-adhesive BMSCs at 6 h. I) Expression of angiogenesis-related genes of HUVECs cultured in the conditioned medium from surface-adhesive BMSCs (VEGF, PDGF, FGF, CD31, α-SMA, and eNOS) on day 3. J-K) Representative images and quantitative assessment of tube-like structure formation of HUVECs cultured in the conditioned medium from encapsulated BMSCs at 6 h. L) Angiogenesis-related gene expression of HUVECs cultured in the conditioned medium from encapsulated BMSC on day 3. (ns = no significant difference, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, n = 3).

Article Snippet: Human umbilical vein endothelial cells (HUVECs, Procell, China) were cultured in the HUVECs complete culture medium (Procell, China) at 37 °C with 5 % CO 2 .

Techniques: Migration, Staining, Wound Healing Assay, Cell Culture, Adhesive, Expressing, Gene Expression

Journal: CNS Neuroscience & Therapeutics

Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke

doi: 10.1111/cns.70345

Figure Lengend Snippet: AEP inhibitor 7,8‐DHF protect HUVECs against oxygen–glucose deprivation by inhibiting tPA‐induced elevated LRP‐1, MMP2, and MMP9. HUVECs treated with 0.5 μM 7,8‐DHF for 24 h followed by OGD 4 h and reoxygen‐glucose plus tPA (500 ng/mL) for another 24 h as the methods part described. (A, B) Western blotting to evaluate the expression of ZO‐1, claudin5, occluding, and JAM‐1. (C) The viability of HUVECs at different time points. (D) AEP enzymatic analysis of HUVECs subjected to 24 h tPA treatment following 7,8‐DHF and OGD. (E, F) Western blotting to evaluate the expression of AEP, p‐TrkB, TrkB, LRP‐1, MMP2, and MMP9. (A) n = 4, (C) n = 6, (D) n = 4, (E) n = 4 per group. Data are presented as mean ± SEM, and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The HUVECs were cultured in complete culture medium for HUVEC (CM‐0122, Procell Life Science & Technology, China).

Techniques: Western Blot, Expressing